This study set out to determine the levels and constituents of the suspended sediment loading in the upper basin of the transboundary Mara River in Kenya and how they relate to the environmental flow requirements of the basin. Catchment degradation in the Upper Mara Basin is causing increased runoff, erosion, and sedimentation of the limited water resources in the basin. The Mara River is an international river shared by Kenya and Tanzania which forms part of the larger Upper Nile River Basin. The Mara River is one of the perennial rivers replenishing the waters of Lake Victoria. In this study, baseline data were collected at Nyang’ores River and Amala River, the tributaries of Mara River.